Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses

The Journal of neuroscience : the official journal of the Society for Neuroscience

Neurotransmitter uptake into synaptic vesicles is mediated by vesicular neurotransmitter transporters. Although these transporters belong to different families, they all are thought to share a common overall topology with an even number of transmembrane domains. Using epitope-specific antibodies and mass spectrometry we show that the vesicular GABA transporter (VGAT) possesses an uneven number of transmembrane domains, with the N terminus facing the cytoplasm and the C terminus residing in the…

Released at: 23.09.2020, written by webmaster_popp