Resolution Matters: Correlating Quantitative Proteomics and Nanoscale-Precision Microscopy for Reconstructing Synapse Identity


For more than a century, the precision at which any protein (or RNA) could be localized in living cells depends on the spatial resolution of microscopy. Light microscopy, even recently benchmarked laser-scanning microscopy, is inherently liable to the diffraction limit of visible light. Electron microscopy that had existed as the only alternative for decades is, in turn, of low throughput and sensitive to processing artefacts. Therefore, researchers have looked for alternative technologies…

Released at: 16.09.2020, written by webmaster_popp